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Proteome Sciences has unprecedented expertise in proteome
analysis, both through in-house capabilities and exclusive research
collaborations with leading academic researchers. A portfolio of proprietary
technologies, ProteoSHOP®, has been developed that
has significantly enhanced the rate of biomarker discovery in disease
diagnosis and therapy. These include 2D Gel Electrophoresis, gel-free
proteomics, mass spectrometry and bioinformatics.
| GEL-BASED PROTEOMICS |
Sample Preparation |
ProteoPrep® |
High solubility and stability during extraction – greater
protein coverage |
2DE Separation |
Narrow pH gradients |
High resolution and increased sensitivity – detection
of low-abundance proteins |
Image Analysis & Differential Display |
PSS®
Progenesis
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Algorithms which enable accurate spot detection
and quantification |
GEL-FREE PROTEOMICS |
Protein Sequence Tags |
PST® |
Gel free – allows hydrophobic membrane proteins to be differentially
analysed in addition to all other proteins. Reduces complexity and
increases protein coverage |
Protein Sequence Tags |
qPST™ |
Uses isotopically labelled pairs of tags that provide relative protein
quantitation |
Tandem Mass Tags |
TMT® |
Different isobaric mass tags in MS/MS allows quantification of differential
expression in up to six samples in a single assay |
In addition, ProteoSHOP® uses most
generic protein analysis techniques available to discover and validate biomarkers.
These include 1D gel electrophoresis, Western blotting, immunostaining,
ELISA, multiple chromatography (MUDPIT), chemical tagging, immunoaffinity
depletion and peptide synthesis for internal standards, TMTcalibrator and AQUA.
KEY TOOLKIT FOR DETECTION OF BIOMARKERS
ProteoPrep® is a registered trademark of Proteome Sciences plc under European Trademark Registration Number 002245587.
ProteoPrep® is also a registered trademark of Proteome Sciences plc in Australia and Japan.
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