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Proteome Sciences has unprecedented expertise in proteome analysis, both through in-house capabilities and exclusive research collaborations with leading academic researchers. A portfolio of proprietary technologies has been developed by PS Biomarker Services that has significantly enhanced the rate of biomarker discovery in disease diagnosis and therapy. These include 2D Gel Electrophoresis, gel-free proteomics, mass spectrometry and bioinformatics.
| GEL-BASED PROTEOMICS |
Sample Preparation |
ProteoPrep® |
High solubility and stability during extraction – greater
protein coverage |
2DE Separation |
Narrow pH gradients |
High resolution and increased sensitivity – detection
of low-abundance proteins |
Image Analysis & Differential Display |
PSS®
Progenesis
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Algorithms which enable accurate spot detection
and quantification |
GEL-FREE PROTEOMICS |
Protein Sequence Tags |
PST® |
Gel free – allows hydrophobic membrane proteins to be differentially
analysed in addition to all other proteins. Reduces complexity and
increases protein coverage |
Protein Sequence Tags |
qPST™ |
Uses isotopically labelled pairs of tags that provide relative protein
quantitation |
Tandem Mass Tags |
TMT® |
Different isobaric mass tags in MS/MS allows quantification of differential
expression in up to six samples in a single assay |
In addition, PS Biomarker Services uses most generic protein analysis techniques available to discover and validate biomarkers. These include 1D gel electrophoresis, Western blotting, immunostaining, ELISA, multiple chromatography (MUDPIT), chemical tagging, immunoaffinity depletion and peptide synthesis for internal standards, TMTcalibrator and AQUA.
KEY TOOLKIT FOR DETECTION OF BIOMARKERS
ProteoPrep® is a registered trademark of Proteome Sciences plc under European Trademark Registration Number 002245587.
ProteoPrep® is also a registered trademark of Proteome Sciences plc in Australia and Japan.
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